Evidence for genetic divergence in ribosomal RNA genes in mycobacteria

Cox, Robert A. ; Katoch, Vishwa M. (1986) Evidence for genetic divergence in ribosomal RNA genes in mycobacteria FEBS Letters, 195 (1-2). pp. 194-198. ISSN 0014-5793

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...

Related URL: http://dx.doi.org/10.1016/0014-5793(86)80159-4


DNA was isolated from Mycobacterium phlei and from M. smegmatis. Each DNA sample was restricted with endonucleases, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose film. Fragments of DNA containing rRNA sequences were identified by means of 125I-labelled rRNA of M. phlei or of M. smegmatis. The distributions of restriction endonuclease sites within the rRNA gene(s) and flanking sequences were found to be characteristic for each of the two species. Hybridizations with heterologous probes indicate that although M. phlei rRNA and M. smegmatis rRNA share regions of sequence homology, they are probably not identical in primary structure. The results suggest that the rRNA genes might prove to be useful taxonomic markers for mycobacteria.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:Mycobacteria rRNA Gene Taxonomy Restriction-endonuclease Site Nucleotide-sequence Homolog
ID Code:16855
Deposited On:16 Nov 2010 13:26
Last Modified:17 May 2016 01:34

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