Parashar, Deepti ; Chauhan, D. S. ; Sharma, V. D. ; Chauhan, Aradhana ; Chauhan, S. V. S. ; Katoch, V. M. (2004) Optimization of procedures for isolation of Mycobacteria from soil and water samples obtained in Northern India Applied and Environmental Microbiology, 70 (6). pp. 3751-3753. ISSN 0099-2240
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Official URL: http://aem.asm.org/cgi/content/abstract/70/6/3751
Related URL: http://dx.doi.org/10.1128/AEM.70.6.3751-3753.2004
Abstract
For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to American Society for Microbiology. |
| ID Code: | 16845 |
| Deposited On: | 16 Nov 2010 13:27 |
| Last Modified: | 17 May 2016 01:33 |
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