Effects of modulating erythrocyte membrane cholesterol on Rho(D) antigen expression

Basu, M. K. ; Flamm, Michael ; Schachter, David ; Bertles, John F. ; Maniatis, Alice (1980) Effects of modulating erythrocyte membrane cholesterol on Rho(D) antigen expression Biochemical and Biophysical Research Communications, 95 (2). pp. 887-893. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...

Related URL: http://dx.doi.org/10.1016/0006-291X(80)90870-0


Alteration of Rho(D)-antigen expression in Rh-positive erythrocytes after modification of membrane fluidity has recently been demonstrated by indirect fluorescence staining. The isolation of D-antigen from Rh-negative erythrocyte membranes has also recently been claimed. We therefore attempted quantification of D-antigen sites in modified Rh-positive and Rh-negative cells by the direct radiolabelled (125I) antibody uptake technique. The cholesterol/phospholipid molar ratio of erythrocytes was modified with resulting membrane-cholesterol enrichment or depletion. 125I-anti-D uptake doubled in enriched Rh-positive erythrocytes, and was decreased in depleted Rh-positive erythrocytes. No change in anti-D uptake could be shown for Rh-negative erythrocytes similarly modified. If the mechanism for enhanced D-antigen expression in cholesterol enriched erythrocytes is vertical displacement of antigen, our inability to unmask the D-antigen in Rh-negative erythrocytes suggests that it is deeply buried in the lipid matrix.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:1683
Deposited On:05 Oct 2010 09:51
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