Purification of a phosphatidylinositol/phosphatidylcholine transfer protein from Neurospora crassa

Basu, Joyoti ; Kundu, Manikuntala ; Chakrabarti, Parul (1992) Purification of a phosphatidylinositol/phosphatidylcholine transfer protein from Neurospora crassa Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1126 (3). pp. 286-290. ISSN 0005-2760

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...

Related URL: http://dx.doi.org/10.1016/0005-2760(92)90242-N

Abstract

This paper reports, for the first time, the purification of a phospholipid transfer protein (PLTP) from a fungus, Neurospora crassa. The protein was purified from the post-microsomal supernatant of N. crassa by successive chromatography on DEAE-cellulose, Sephadex-G75 and PBE 94 (pH 4-7). The purified protein (Mr38 000) was found to transfer phosphatidylinositol preferentially over phosphatidylcholine, like the PLTP from the yeast, Saccharomyces cerevisiae. PC transfer was completely inhibited by inactivation of free amino groups or tryptophan residues. Surprisingly, the protein did not cross-react with antibodies against the bovine brain P1TP. The cellular content of the protein was maximal during the logarithmic phase of growth. However, no direct correlation between the content of the protein and PC transfer activity could be demonstrated.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Phospholipid Transfer Protein; Protein Purification; (N. crassa)
ID Code:1682
Deposited On:05 Oct 2010 09:48
Last Modified:17 Jan 2011 08:14

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