Molecular conformation of gonadoliberin using two-dimensional NMR spectroscopy

Chary, Kandala V. R. ; Srivastava, Sudha ; Hosur, Ramakrishna V. ; Roy, Kunal B. ; Govil, Girjesh (1986) Molecular conformation of gonadoliberin using two-dimensional NMR spectroscopy European Journal of Biochemistry, 158 (2). pp. 323-332. ISSN 0014-2956

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Complete resonance assignments of the proton NMR spectrum of gonadoliberin (in its native amide and free acid forms) have been obtained using two-dimensional nuclear magnetic resonance spectroscopy under three different environmental conditions, namely, dimethyl sulphoxide solution, aqueous solution and lipid-bound form in model membranes. The proton chemical shifts in the three cases have been compared to derive information about inherent conformational characteristics of the molecule. It has been inferred that the molecule possesses no short-range or long-range order under any of the three solvent conditions. However, there is a nonspecific increase in the linewidths when gonadoliberin is bound to model membranes, indicating a reduced internal motion in the molecule due to lipid-peptide interactions.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
ID Code:16730
Deposited On:15 Nov 2010 14:12
Last Modified:17 May 2016 01:27

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