Determination of ethambutol MICs for Mycobacterium tuberculosis and Mycobacterium avium isolates by resazurin microtitre assay

Jadaun, G. P. S. ; Agarwal, Chhaya ; Sharma, Hirdesh ; Ahmed, Zafar ; Upadhyay, Prashant ; Faujdar, Jaya ; Gupta, Anuj Kumar ; Das, Ram ; Gupta, Pushpa ; Chauhan, D. S. ; Sharma, V. D. ; Katoch, V. M. (2007) Determination of ethambutol MICs for Mycobacterium tuberculosis and Mycobacterium avium isolates by resazurin microtitre assay Journal of Antimicrobial Chemotherapy, 60 (1). pp. 152-155. ISSN 0305-7453

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Official URL: http://jac.oxfordjournals.org/content/60/1/152.abs...

Related URL: http://dx.doi.org/10.1093/jac/dkm117

Abstract

Objectives: To test susceptibilities of Mycobacterium tuberculosis (MTB) isolates to ethambutol by the Lowenstein-Jensen (LJ) proportion method and resazurin microtitre assay (REMA) and to evaluate REMA for the determination of ethambutol MICs for MTB and Mycobacterium avium isolates. Methods: A total of 50 MTB and 20 M. avium isolates were tested to determine the MICs of ethambutol by REMA and agar dilution method. MTB isolates were also tested by the LJ proportion method. Results: REMA provided ethambutol susceptibility results for all the isolates within 8-9 days. For MTB isolates, REMA showed 96.7% sensitivity, 100.0% specificity and 98.0% accuracy when LJ proportion results were taken as 'gold standard'. For both MTB and M. avium isolates, the MICs determined by REMA were lower than those determined in agar medium, indicating that MIC values determined by REMA are closer to the actual MICs for the isolates. Conclusions: REMA can be used as a rapid and inexpensive method for mycobacterial drug susceptibility testing against ethambutol. In comparison with the agar method, the MICs determined by REMA can more accurately be correlated with achievable plasma concentrations of antimycobacterial agents.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
Keywords:Mycobacteria; Drug Susceptibility; M. Tuberculosis; M. Avium
ID Code:16681
Deposited On:15 Nov 2010 13:28
Last Modified:17 May 2016 01:25

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