Alanine check points in HNN and HN(C)N spectra

Chatterjee, Amarnath ; Kumar, Ashutosh ; Hosur, Ramakrishna V. (2006) Alanine check points in HNN and HN(C)N spectra Journal of Magnetic Resonance, 181 (1). pp. 21-28. ISSN 1090-7807

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Rapid resonance assignment is a key requirement in structural genomics research by NMR. In this context we present here two new pulse sequences, namely, HNN-A and HN(C)N-A that have been developed by simple modification of the previously described pulse sequences, HNN and HN(C)N [S.C. Panchal, N.S. Bhavesh, R.V. Hosur, Improved 3D triple resonance experiments, HNN and HN(C)N, for HN and 15N sequential correlations in (13C, 15N) labeled proteins: application to unfolded proteins, J. Biomol. NMR, 20 (2001) 135-147]. These increase the number of start/check points in HNN and/or HN(C)N spectra and hence help in pacing up resonance assignment in proteins.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:HNN-A; HN(C)N-A; Check Points; Triplets of Residues; Band Selective Pulse
ID Code:16633
Deposited On:15 Nov 2010 13:32
Last Modified:03 Jun 2011 08:45

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