NMR insights into dynamics regulated target binding of DLC8 dimer

Krishna Mohan, P. M. ; Hosur, Ramakrishna V. (2007) NMR insights into dynamics regulated target binding of DLC8 dimer Biochemical and Biophysical Research Communications, 355 (4). pp. 950-955. ISSN 0006-291X

Full text not available from this repository.

Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1016/j.bbrc.2007.02.072


Conformational dynamics play a crucial role in biological function. Dynein light chain protein (DLC8) acts as a cargo adaptor, and exists as a dimer under physiological conditions and dissociates into monomer below pH 4. In the present NMR study, we identified some dynamic residues in the dimer using chemical shift perturbation approach by applying small pH change. As evidenced by gel filtration and CD studies, this small pH change does not alter the globular structural features of the protein. In fact, these changes result in small local stability perturbations as monitored using temperature dependence of amide proton chemical shifts, and influence the dynamics of the dimer substantially. Further, interaction studies of the protein with a peptide containing the recognition motif of cargo indicated that the efficacy of peptide binding decreases when the pH is reduced from 7 to 6. These observations taken together support the conception that dynamics can regulate cargo binding/trafficking by the DLC8 dimer.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Dynein Light Chain Protein; Cargo Trafficking; Gel Filtration; Nuclear Magnetic Resonance; Temperature Coefficients; pH Sensitivity; Circular Dichroism
ID Code:16628
Deposited On:15 Nov 2010 13:32
Last Modified:03 Jun 2011 08:46

Repository Staff Only: item control page