Chaturvedi, M. M. ; Kanungo, M. S. (1985) Analysis of chromatin of the brain of young and old rats by nick-translation Biochemical and Biophysical Research Communications, 127 (2). pp. 604-609. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1016/S0006-291X(85)80203-5
Abstract
Chromatin of the brain of young (22-23 week) and old (118-119 week) rats has been analysed by nick-translation reaction following its digestion by DNaseI, EcoRI, MspI and HpaII. The incorporation of (3H)-dTMP in the old is only about 50 percent of that of the young. The difference in the incorporation following digestion of nuclei by MspI and HpaII that quantitate the degree of methylation of internal cytosines in the 5' CCGG 3' sequences, is nearly two-fold higher in the old. These data indicate that the chromatin undergoes increasing condensation as a function of age. One of the contributory factors may be increasing methylation of DNA. This may decrease the active fraction of chromatin.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 16362 |
| Deposited On: | 15 Nov 2010 13:47 |
| Last Modified: | 03 Jun 2011 09:04 |
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