Effect of altered polar headgroup of phosphatidylethanolamines on transbilayer aminophospholipid distribution in sonicated vesicles

Kumar, Ajay ; Gupta, C. M. (1983) Effect of altered polar headgroup of phosphatidylethanolamines on transbilayer aminophospholipid distribution in sonicated vesicles Biochimica et Biophysica Acta (BBA) - Biomembranes, 730 (1). pp. 1-9. ISSN 0005-2736

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...

Related URL: http://dx.doi.org/10.1016/0005-2736(83)90309-7

Abstract

The transbilayer aminophospholipid distributions in small unilamellar vesicles comprising of phosphatidylethanolamine or its analogs (bearing modifications in the polar headgroup) and egg phosphatidylcholine were ascertained using trinitrobenzenesulfonic acid as external membrane probe. These vesicles, containing 10-30 mol % phosphatidylethanolamine or its analogs, were formed by sonication and fractionated by centrifugation. Phosphatidylethanolamine at low concentrations (10 mol %) preferentially localized in the outer monolayer. This preference appeared to be reversed at higher phosphatidylethanolamine concentrations (30 mol %). Unlike this finding, phosphatidylethanolamine bearing ethyl, phenyl and benzyl substituents at the carbon atom adjacent to the amino group distributed mainly in the outer surface irrespective of their concentrations. Similar results were obtained when the phosphate and amino groups were separated by three methylene residues. These observations suggest that the effective polar headgroup volume and/or hydrogen-bonding capacity of phospholipids are the important factors that determine their distribution in small unilamellar vesicles.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Phospholipid Distribution; Phosphatidylethanolamine Analog; Aminophospholipid; Headgroup; (Membrane Vesicle)
ID Code:16006
Deposited On:16 Nov 2010 13:34
Last Modified:17 May 2016 00:50

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