Characteristics of transport of L-leucine and glycine in pea protoplasts

Dureja, Inam ; Guha-Mukherjee, Sipra ; Prasad, Rajendra (1984) Characteristics of transport of L-leucine and glycine in pea protoplasts Journal of Experimental Botany, 35 (7). pp. 1022-1031. ISSN 0022-0957

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Official URL: http://jxb.oxfordjournals.org/cgi/content/abstract...

Related URL: http://dx.doi.org/10.1093/jxb/35.7.1022

Abstract

The uptake of L - leucine and glycine into pea protoplasts was studied under various conditions. The uptake of both L - leucine and glycine was pH dependent with the optimal pH being 4.0 and 5.0 for L - leucine and glycine, respectively. A kinetic study of L - leucine uptake showed that uptake is multiphasic; Km values of different phases were 1.1 mol m-3, 33.3 mol m-3 and 100 mol m3. A similar analysis for glycine at a concentration range of 0.1-10 mol m-3 also showed a multiphasic transport system for it. The uptake of L - leucine at lower concentrations(between 0.1 - 2.0 mol m-3) was energy dependent, since arsenate, azide, dinitrophenol and iodoacetate inhibited the uptake. However, the uptake of L - leucine was not inhibited by ouabain at any concentration of L - leucine employed. The uptake of glycine was not inhibited by any of these inhibitors suggesting that glycine uptake was not mediated by an active process.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
Keywords:Pea Protoplast; L-leucine; Glycine Transport; Active Transport
ID Code:15646
Deposited On:13 Nov 2010 12:47
Last Modified:21 Feb 2011 06:32

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