Mechanism of L-lysine transport by pea protoplasts

Dureja, I. ; Guha-Mukherjee, Sipra ; Prasad, Rajendra (1986) Mechanism of L-lysine transport by pea protoplasts Journal of Experimental Botany, 37 (4). pp. 549-555. ISSN 0022-0957

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L - Lysine uptake was studied in pea protoplasts to characterize the transport process. The uptake was pH dependent with optimum at pH 5.8. A kinetic analysis of uptake showed that L- lyslne uptake was biphasic. The respiratory inhibitors, sodium arsenate, azide, iodoacetate and 2, 4, dinitrophenol, inhibited the uptake of L- lysine at a final concentration of 0.1 mol m -3 suggesting it to be mediated in part by an active process. Competitive inhibition of L- lysine uptake by only L - arglnine and of L- leucine and glycine uptake by several amino acids indicated that L- lysine uptake occurs via a specific system whereas the uptake of L- leucine and glycine was mediated through a relatively non - specific permease.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
Keywords:Pea Protoplasts; L-lysine Transport; Active Transport; Specific System
ID Code:15642
Deposited On:13 Nov 2010 12:47
Last Modified:21 Feb 2011 06:30

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