Sahdev, Sudhir ; Taneja, Tarvinder K. ; Mohan, Manjari ; Sah, Nand K. ; Khar, Ashok K. ; Hasnain, Seyed E. ; Athar, Mohammad (2003) Baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway Biochemical and Biophysical Research Communications, 307 (3). pp. 483-490. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1016/S0006-291X(03)01224-5
Abstract
In this study we report that the baculovirus p35 anti-apoptotic protein prevents cell death by quenching free radicals at a very upstream step in the apoptotic pathway. Mitochondria of activated rat peritoneal macrophages as well as Spodoptera frugiperda (Sf9) insect cells, following treatment with oxidants, H2O2/UVB irradiation, release cytochrome c followed by activation of caspase-3. Transfection of macrophages/Sf9 cells with a construct carrying the p35 gene under the CMV/HSP promoters resulted in p35 expression and consequent arrest of oxidative stress-induced apoptosis. p35 expression also inhibited cytochrome c release from the mitochondria of oxidant-exposed cells and blocked caspase-3 activation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Baculovirus; p35; Apoptosis; Oxidative Stress; Cytochrome c Release; Caspase-inhibition |
| ID Code: | 15584 |
| Deposited On: | 13 Nov 2010 12:53 |
| Last Modified: | 03 Jun 2011 06:36 |
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