A 500 MHz proton NMR study of the interaction of the tripeptide Lys-Tyr-Lys with the tetradeoxynucleotides d-CpCpGpG and d-CpGpCpG

Barthwal, Ritu ; Kukreti, Shrikant ; Mujeeb, Anwer ; Govil, Girjesh (1993) A 500 MHz proton NMR study of the interaction of the tripeptide Lys-Tyr-Lys with the tetradeoxynucleotides d-CpCpGpG and d-CpGpCpG Magnetic Resonance Materials in Physics, Biology and Medicine, 1 (3-4). pp. 145-157. ISSN 0968-5243

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Official URL: http://www.springerlink.com/content/q547334h0228mu...

Related URL: http://dx.doi.org/10.1007/BF01769417

Abstract

Two dimensional proton NMR experiments at 500 MHz show that d-CpCpGpG exists as a right handed B-DNA structure in D2O solution with both sugars in O1' endo and glycosidic bond angle in anticonformation. On complexation with tripeptide Lys-Tyr-Lys, it forms a well characterized complex with two identical strands. The Tyr ring protons shift upfield by about 0.07p.p.m. at 285 K due to stacking with base pairs. This is accompanied by changes in chemical shift of base protons and the melting temperatureT m with practically no change in conformation of tetranucleotide. All changes in chemical shift decrease with temperature. Interaction of d-CpGpCpG with Lys-Tyr-Lys leads to a comparatively large upfield shift of Tyr ring protons, being ~0.14 p.p.m. at 285 K followed by stabilisation of double helix. A comparison of the interaction of tripeptide with different di- and tetranucleotides shows that the interaction decreases in the order d-GpCpGpC > d-CpGpCpG > d-CpCpGpG > d-GpC > d-CpG apparently suggesting that Tyr ring stacks preferentially in 5'-GpC 3' site. Such findings have important implications in protein-nucleic acid interactions.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Deoxytetranucleotide d-cpcpgpg; Deoxytetranucleotide d-cpgpcpg; Conformation by 2d nmr; Stacking Interaction/Intercalation; Tripeptide lys-tyr-lys Interaction with Deoxy-tetranucleotides
ID Code:15497
Deposited On:13 Nov 2010 09:04
Last Modified:14 Feb 2011 09:43

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