Magnetic resonance methods for studying intact spermatozoa

Phadke, Ratna S. ; Srivastava, Sudha ; Govil, Girjesh (1990) Magnetic resonance methods for studying intact spermatozoa Journal of Biosciences, 15 (3). pp. 125-134. ISSN 0250-5991

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Motility is used as a routine parameter for assessing spermatozoa activity. The quality rating techniques adopted are based on electron or optical microscopy. However, these methods depend on gross structural and dynamical features of sperm cells and do not provide information on metabolic activity of intact cells. Lately, biochemical assays have become popular. Such methods are cumbersome and destroy the samples. Magnetic resonance methods offer a non-invasive method for studies on intact sperms. We have investigated respiration, maturation andin vitro capacitation of sperms from human ejaculates and sperms extracted from goat reproductive organ using electron spin resonance spin labelling and [31P] nuclear magnetic resonance methods. These studies clearly establish the advantages of magnetic resonance in studies related to metabolic activity of sperms.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Sperms; ESR Spin Labelling; [31P] NMR; Human Semen; Goat Reproductive Organ; Electron Transport Chain; Cold Shock
ID Code:15492
Deposited On:13 Nov 2010 09:04
Last Modified:17 May 2016 00:23

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