Evidence for A+(anti)-G(syn) mismatched base-pairing in d-GGTAAGCGTACC

Sau, Apurba K. ; Chary, Kandala V. R. ; Govil, Girjesh ; Chen, Chang-qing ; Howard, Frank B. ; Todd Miles, H. (1995) Evidence for A+(anti)-G(syn) mismatched base-pairing in d-GGTAAGCGTACC FEBS Letters, 377 (3). pp. 301-305. ISSN 0014-5793

PDF - Publisher Version

Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...

Related URL: http://dx.doi.org/10.1016/0014-5793(95)01362-8


Two-dimensional NMR spectroscopy has been used to study the structure and hydrogen bonding scheme of A:G mismatched base pairing in d-GGTAAGCGTACC at pH 5.8. Under the conditions of our study, the molecule forms a B-DNA helix, with the mismatched bases in the A+(anti)-G(syn) conformation. The adenosine exists in the protonated form. The NOESY spectrum in 90% H2O + 10% 2H2O has been used to assign all observable imino and amino protons including those involved in the A+(anti)-G(syn) base pair. Both the proton donors in the A:G mismatched inter-base hydrogen bonding are situated on adenosine.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:A:G Base Pairing; Mismatch Base Pair; DNA; Nuclear Overhauser Enhancement Spectroscopy; Clean Total Correlation Spectroscopy
ID Code:15383
Deposited On:13 Nov 2010 09:07
Last Modified:17 May 2016 00:17

Repository Staff Only: item control page