Sau, Apurba K. ; Chary, Kandala V. R. ; Govil, Girjesh ; Chen, Chang-qing ; Howard, Frank B. ; Todd Miles, H. (1995) Evidence for A+(anti)-G(syn) mismatched base-pairing in d-GGTAAGCGTACC FEBS Letters, 377 (3). pp. 301-305. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...
Related URL: http://dx.doi.org/10.1016/0014-5793(95)01362-8
Abstract
Two-dimensional NMR spectroscopy has been used to study the structure and hydrogen bonding scheme of A:G mismatched base pairing in d-GGTAAGCGTACC at pH 5.8. Under the conditions of our study, the molecule forms a B-DNA helix, with the mismatched bases in the A+(anti)-G(syn) conformation. The adenosine exists in the protonated form. The NOESY spectrum in 90% H2O + 10% 2H2O has been used to assign all observable imino and amino protons including those involved in the A+(anti)-G(syn) base pair. Both the proton donors in the A:G mismatched inter-base hydrogen bonding are situated on adenosine.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
| Keywords: | A:G Base Pairing; Mismatch Base Pair; DNA; Nuclear Overhauser Enhancement Spectroscopy; Clean Total Correlation Spectroscopy |
| ID Code: | 15383 |
| Deposited On: | 13 Nov 2010 09:07 |
| Last Modified: | 17 May 2016 00:17 |
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