Magnetic resonance spectroscopy of intact spermatozoa

Govil, Girjesh ; Phadke, Ratna S. ; Srivastava, Sudha ; Hegde, Umashashi C. ; Fernandes, Elvyra J. (1992) Magnetic resonance spectroscopy of intact spermatozoa Spectrochimica Acta Part A: Molecular Spectroscopy, 48 (11-12). pp. 1759-1766. ISSN 0584-8539

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NMR and ESR techniques have been used to elucidate the cellular metabolism and maturation of goat spermatozoa. Due to electron transport chains in the mitochondrial part of the sperm, these cells reduce nitroxide spin labels such as TEMPO and the ESR signal intensity is a good measure of the reducing power of live cells. The use of 31P NMR helps in detecting changes in intracellular pH during metabolism as well as cellular capacity to generate ATP, while 13C NMR has been used to monitor metabolites generated by cells derived from different regions. The maturation of spermatozoa as it passes through the epididymal track has been characterized by magnetic resonance spectroscopy.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:15374
Deposited On:13 Nov 2010 09:11
Last Modified:13 Nov 2010 09:11

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