A novel loop structure observed in d-GAATTCCCGAATTC by 2D NMR

Hosur, R. V. ; Sheth, Anu ; Chary, K. V. R. ; Ravikumar, M. ; Govil, Girjesh ; Zu-kun, Tan ; Todd Miles, H. (1986) A novel loop structure observed in d-GAATTCCCGAATTC by 2D NMR Biochemical and Biophysical Research Communications, 139 (3). pp. 1224-1232. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1016/S0006-291X(86)80308-4


Sequential resonance assignments of the non exchangeable base and sugar protons in d-GAATTCCCGAATTC have been obtained using two dimensional NMR experiments at 500 MHz. The chemical shifts and the NOEs have been used to determine the structure in the base-pair mismatch region which is located in the central portion of the molecule. It is observed that the molecule adopts a novel unsymmetrical loop structure in this section which is characterised by sugar geometries which are significantly different compared to the rest of the molecule. The base-paired portion of the molecule conforms to a right handed B-DNA type of structure.

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Deposited On:13 Nov 2010 09:13
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