Pisum sativum contains a factor with strong homology to eIF5B

Rasheedi, Sheeba ; Ghosh, Sudip ; Suragani, Madhuri ; Tuteja, Narendra ; Sopory, Sudhir K. ; Hasnain, Seyed E. ; Ehtesham, Nasreen Z. (2007) Pisum sativum contains a factor with strong homology to eIF5B Gene, 399 (2). pp. 144-151. ISSN 0378-1119

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S03781...

Related URL: http://dx.doi.org/10.1016/j.gene.2007.05.004

Abstract

Immunoscreening of a cDNA expression library, prepared from 7 days old young shoots of pea (Pisum sativum), identified a novel gene comprising of 2586 bp open reading frame (ORF) with 381 bp and 532 bp 5' and 3'untranslated regions (UTRs), respectively. Sequence analysis of this gene, termed as PeIF5B, revealed striking homology to eukaryotic translation initiation factor eIF5B - a sequence homologue of prokaryotic translation initiation factor IF2. Southern blot analyses indicated that PeIF5B exists as a single copy gene in P. sativum genome. Northern blot hybridization revealed the presence of a 7 kb transcript in pea plant. In vitro translation using rabbit reticulocyte lysate system yielded a protein corresponding to 116 kDa which was higher than the calculated value of 96 kDa. Phylogenetic analyses of PeIF5B placed it closer to eIF5B from yeast, human and Drosophila. Pfam domain search analysis pointed to its likely role as a translation initiation factor. The presence of an eIF5B-like factor in a plant system will aid in better understanding of the mechanism of translation initiation in plants.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:IF2; GTP-binding Domain; EF-Tu-D2; Phylogenetic Tree
ID Code:15299
Deposited On:13 Nov 2010 12:59
Last Modified:14 Mar 2012 11:24

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