Akhter, Yusuf ; Tundup, Smanla ; Hasnain, Seyed E. (2007) Novel biochemical properties of a CRP/FNR family transcription factor from Mycobacterium tuberculosis International Journal of Medical Microbiology, 297 (6). pp. 451-457. ISSN 1438-4221
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S14384...
Related URL: http://dx.doi.org/10.1016/j.ijmm.2007.04.009
Abstract
Cyclic AMP (cAMP) receptor protein (CRP)/fumarate nitrate reductase regulator (FNR) family proteins are actively associated with defense against low oxygen stress, starvation and extreme temperature conditions. They are DNA-binding proteins and regulate target genes carrying the regulatory CRP/FNR cognate nucleotide sequence elements. Recombinant protein encoded by the Mycobacterium tuberculosis ORF Rv3676, a putative CRP/FNR regulator, was purified from Escherichia coli and was found to exist as dimer, devoid of any metal cation cofactor. Purified rRv3676 exhibited cAMP binding in a concentration-dependent manner. At lower concentrations of cAMP (6-10 μM) rRv3676 shows positive cooperativity; at 10 μM cAMP the protein exists in the most open conformation. rRv3676 could bind specifically to the putative CRP/FNR nucleotide sequence elements as evident from electrophoretic mobility shift assay.
Item Type: | Article |
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Source: | Copyright of this article belongs to Urban and Fischer Verlag. |
Keywords: | Hypoxia; Starvation; Cyclic AMP; Rv3676; Transcription Factor; Gene Regulation |
ID Code: | 15260 |
Deposited On: | 13 Nov 2010 13:02 |
Last Modified: | 03 Jun 2011 06:17 |
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