Site-specific gene delivery in vivo through engineered Sendai viral envelopes

Ramani, Komal ; Hassan, Quamarul ; Venkaiah, Betapudi ; Hasnain, Seyed E. ; Sarkar, Debi P. (1998) Site-specific gene delivery in vivo through engineered Sendai viral envelopes Proceedings of the National Academy of Sciences of the United States of America, 95 (20). pp. 11886-11890. ISSN 0027-8424

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Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cells of BALB/c mouse in vivo. The membrane fusion-mediated high efficiency of gene transfer to liver cells was ascertained following a critical evaluation of the level of the DNA, mRNA, and relevant proteins. Furthermore, the involvement of viral glycoprotein both as a unique natural ligand and as a membrane fusogen could lead to preferential transfection of parenchymal cell types of liver. The integration of transgenes in the mouse chromosomal DNA and its stable expression up to 4 mo after single i.v. administration of this gene carrier has bolstered its efficiency and novelty. Moreover, the F-virosomes did not elicit significant humoral immune response against the fusion protein in the injected animal. The findings reported here open up the possibility for considering "F-virosomes" as a promising "vehicle" for site-specific DNA delivery in gene therapy.

Item Type:Article
Source:Copyright of this article belongs to National Academy of Sciences, USA.
ID Code:15190
Deposited On:13 Nov 2010 06:38
Last Modified:17 May 2016 00:07

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