Genetic analysis of traditional and evolved Basmati and non-Basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers

Nagaraju, J. ; Kathirvel, M. ; Ramesh Kumar, R. ; Siddiq, E. A. ; Hasnain, Seyed E. (2002) Genetic analysis of traditional and evolved Basmati and non-Basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers Proceedings of the National Academy of Sciences of the United States of America, 99 (9). pp. 5836-5841. ISSN 0027-8424

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Official URL: http://www.pnas.org/content/99/9/5836.abstract

Related URL: http://dx.doi.org/10.1073/pnas.042099099

Abstract

The objective of the present study was to make use of efficient molecular marker systems to reveal genetic relationships in traditional and evolved Basmati (EB) and semidwarf non-Basmati (NB) rice varieties. A subset of three rice groups was analyzed by using 19 simple sequence repeat (SSR) loci and 12 inter-SSR-PCR primers. A total of 70 SSR alleles and 481 inter-SSR-PCR markers were revealed in 24 varieties from the three groups. The lowest genetic diversity was observed among the traditional Basmati varieties, whereas the EB varieties showed the highest genetic diversity by both the marker assays. The results indicated that the subset of aromatic rice varieties analyzed in the present study is probably derived from a single land race. The traditional Basmati (TB) and semidwarf NB rice varieties used in the present study were clearly delineated by both marker assays. A number of markers, which could unambiguously distinguish the TB varieties used in the present study from the evolved and NB rice varieties, were identified. The potential use of these markers in Basmati rice-breeding programs and authentication of TB varieties used in the present study are envisaged.

Item Type:Article
Source:Copyright of this article belongs to National Academy of Sciences, USA.
ID Code:15098
Deposited On:13 Nov 2010 06:47
Last Modified:17 May 2016 00:03

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