Occurrence and biosynthesis of auxin in protonema of the moss Funaria hygrometrica

Jayaswal, Radheshyam K. ; Johri, Man Mohan (1985) Occurrence and biosynthesis of auxin in protonema of the moss Funaria hygrometrica Phytochemistry, 24 (6). pp. 1211-1214. ISSN 0031-9422

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00319...

Related URL: http://dx.doi.org/10.1016/S0031-9422(00)81103-6


We have investigated the presence of auxin and the ability of chloronema cells to synthesize indole-3-acetic acid (IAA) in axenic protonema cell cultures of the moss Funaria hygrometrica. The endogenous level of auxin activity was 4 and 7µ g-IAA equivalents/kg in caulonema and chloronema cell types, respectively. Based on an indole-α -pyrone fluorometric assay, the level of putative IAA was observed to be 5.0 and 1.9.µ g/kg in caulonema and chloronema cells, respectively. [3H]Tryptophan was metabolized into IAA via the indole-pyruvate pathway by intact chloronema cells and also by the cell free homogenates. More [3H]IAA accumulated when homogenates from cells pre-grown at low cell densities (< 0.5 mg/ml) as compared to those at high cell densities ( > 0.5 mg/ml) were used. Since the activities of peroxidase and IAA-oxidase are known to be high at high cell densities, the lack of accumulation of radioactivity in IAA at high densities can be attributed to a high level of IAA-oxidizing enzymes. Our results suggest a possible relationship between IAA accumulation and caulonema differentiation.

Item Type:Article
Source:Copyright of this article belongs to Phytochemical Society of Europe.
Keywords:Funaria hygrometrica; Bryidae; Moss; Protonema; Auxin; Cell-density Dependence
ID Code:14955
Deposited On:13 Nov 2010 13:14
Last Modified:19 Feb 2011 03:22

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