Altered levels of Gq activity modulate axonal pathfinding in Drosophila

Ratnaparkhi, Anuradha ; Banerjee, Santanu ; Hasan, Gaiti (2002) Altered levels of Gq activity modulate axonal pathfinding in Drosophila Journal of Neuroscience, 22 (11). pp. 4499-4508. ISSN 0270-6474

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A majority of neurons that form the ventral nerve cord send out long axons that cross the midline through anterior or posterior commissures. A smaller fraction extend longitudinally and never cross the midline. The decision to cross the midline is governed by a balance of attractive and repulsive signals. We have explored the role of a G-protein, Gαq, in altering this balance in Drosophila. A splice variant of Gαq, dgqα3, is expressed in early axonal growth cones, which go to form the commissures in the Drosophila embryonic CNS. Misexpression of a gain-of-function transgene of dgqα3 (AcGq3) leads to ectopic midline crossing. Analysis of the AcGq3 phenotype in roundabout and frazzled mutants shows that AcGq3 function is antagonistic to Robo signaling and requires Frazzled to promote ectopic midline crossing. Our results show for the first time that a heterotrimeric G-protein can affect the balance of attractive versus repulsive cues in the growth cone and that it can function as a component of signaling pathways that regulate axonal pathfinding.

Item Type:Article
Source:Copyright of this article belongs to Society for Neuroscience.
Keywords:dgq; Robo; Frazzled; Netrins; G-protein; Midline; Axon guidance
ID Code:14849
Deposited On:12 Nov 2010 13:28
Last Modified:16 May 2016 23:49

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