Glutathione modulates lipid composition of human colon derived HT-29 cells

Madesh, M. ; Benard, O. ; Balasubramanian, K. A. (1998) Glutathione modulates lipid composition of human colon derived HT-29 cells International Journal of Biochemistry & Cell Biology, 30 (12). pp. 1345-1352. ISSN 1357-2725

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S13572...

Related URL: http://dx.doi.org/10.1016/S1357-2725(98)00097-1

Abstract

Glutathione (GSH) is important in maintaining intracellular thiol status. The present study looked at the effect of GSH depletion on lipid composition of colon-derived HT-29 cells. GSH was depleted in HT-29 cells by incubation either with buthionine-S, R-sulfoximine (BSO) or diethylmaleate (DEM). GSH was restored during early periods of cell growth by supplementation of growth medium with either GSH ester or N-acetyl cysteine (NAC). Lipids were analysed following GSH depletion and supplementation. Among the neutral lipids, an increase in free cholesterol and diacylglycerol and decrease in cholesteryl ester and triacylglycerol were seen in GSH-depleted cells as compared to control cells. There were no detectable free fatty acids either in control or GSH-depleted cells. Among the phospholipids, a decrease in phosphatidylcholine and phosphatidylinositol and an increase in phosphatidylethanolamine were observed. These changes were almost completely reversed by supplementation of BSO-treated cells with GSH ester and partially reversed by N-acetyl cysteine. These results suggest that the GSH status of the cell plays an important role in the lipid composition of the cells.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Glutathione; N-acetyl Cysteine; BSO; DEM; HT-29 Cells; Lipids
ID Code:1463
Deposited On:05 Oct 2010 12:27
Last Modified:14 May 2011 05:01

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