Synthetic hydrogel microspheres as substrata for cell adhesion and growth

Shivakumar, K. ; Renuka Nair, R. ; Jayakrishnan, A. ; Chithambara Thanoo, B. ; Kartha, C. C. (1989) Synthetic hydrogel microspheres as substrata for cell adhesion and growth In Vitro Cellular and Developmental Biology: Plant, 25 (4). pp. 353-357. ISSN 1054-5476

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Official URL: http://www.springerlink.com/content/p8p28413v24051...

Related URL: http://dx.doi.org/10.1007/BF02624598

Abstract

Cross-linked poly(methyl methacrylate) (PMMA) microspheres were subjected to alkaline hydrolysis to obtain hydrophilic microspheres having carboxyl residues distributed throughout the matrix. These microspheres were found to support the growth of human skin fibroblasts and human heart and lung cells. Further, fibroblasts grown on them were found to be comparable with those grown on the commercial tissue culture plate with respect to [14C]amino acid uptake and incorporation into proteins. The hydrolyzed PMMA microspheres may find application as a microcarrier for cell culture.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Hydrogel Microspheres; Cell Culture; Microcarrier Culture
ID Code:13915
Deposited On:12 Nov 2010 14:29
Last Modified:03 Jun 2011 10:03

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