Balasubramanian, K. A. ; Bachhawat, B. K. (1975) Purification, properties and glycoprotein nature of arylsulfatase A from sheep brain Biochimica et Biophysica Acta (BBA) - Enzymology, 403 (1). pp. 113-121. ISSN 0005-2744
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...
Related URL: http://dx.doi.org/10.1016/0005-2744(75)90014-5
Abstract
A simple and rapid method for the purification of arylsulfatase A (EC 3.1.6.1) from sheep brain has been developed. This includes the concanavalin A-Sepharose affinity chromatography and the pH-dependent polymerization and depolymerization of the enzyme. By these methods a homogeneous enzyme was obtained and the enzyme was purified 7180-fold. Sheep brain arylsulfatase A has been shown to be a glycoprotein containing 25% neutral sugar and 0.5% sialic acid. The constituent neutral sugars were identified as glucose and mannose.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 1378 |
| Deposited On: | 05 Oct 2010 12:40 |
| Last Modified: | 14 May 2011 05:30 |
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