Effect of oxidant exposure on monkey intestinal brush-border membrane

Nalini, S. ; Ahamed Ibrahim, S. ; Balasubramanian, K. A. (1993) Effect of oxidant exposure on monkey intestinal brush-border membrane Biochimica et Biophysica Acta (BBA) - Biomembranes, 1147 (2). pp. 169-176. ISSN 0005-2736

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...

Related URL: http://dx.doi.org/10.1016/0005-2736(93)90001-G

Abstract

This study looks at the effect of oxidant exposure on changes in structural components and functional properties of monkey intestinal brush-border membrane vesicles (BBMV). These membranes were found resistant to iron-dependent lipid peroxidation as judged by measurement of various parameters such as formation of malonaldehyde (MDA) and conjugated diene and depletion of total arachidonic acid, tocopherol and membrane-associated protein thiol groups. Free radicals generated by thermal decomposition of 2,2'-azobis(2-amidinopropane) dihydrochloride (ABAP) which does not require iron, were capable of inducing lipid peroxidation in this membrane. Fluorescence polarisation studies used to assess the physical state of the membrane lipids after exposure to various free radical generating systems showed that ABAP could decrease the fluidity of BBMV whereas other systems had no effect. Exposure of BBMV to ABAP or cumene hydroperoxide decreased the glucose and amino acid transport. BBMV had a high content of nonesterified fatty acids as part of the total lipids and removal of these free fatty acids by treatment with fatty acid free albumin made the membranes susceptible to iron-dependent peroxidation. These studies suggest that intestinal epithelial cell membranes are resistant to iron-dependent lipid peroxidation due to the presence of membrane-associated free fatty acids. Possibly lipid peroxidation may play a less significant role in damage to these cells.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Lipid Peroxidation; Fluidity; Transport; Brush-border Membrane; (Intestine)
ID Code:1370
Deposited On:05 Oct 2010 12:41
Last Modified:14 May 2011 05:17

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