Cell surface molecules (NCAM and LI) in intrastriatal transplants of embryonic mesencephalon in rats

Gopinath, G. ; Sable, V. ; Sailaja, K. ; Tandon, P. N. (1996) Cell surface molecules (NCAM and LI) in intrastriatal transplants of embryonic mesencephalon in rats Neuroscience, 73 (1). pp. 161-169. ISSN 0306-4522

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/030645...

Related URL: http://dx.doi.org/10.1016/0306-4522(96)00037-1


Cell surface molecules, NCAM and L1, reported to have a role in synaptogenesis, growth and fasciculation of the neurites in the brain, were traced in the embryonic nigral transplants in the host striatum of adult rats. Substantia nigra of five, 15 and 25 postnatal days were also examined for the same molecules. Tyrosine hydroxylase label was used as a marker to localize the nigral neurons and glial fibrillary acidic protein to detect if glial scar present. In the control as well as transplants large neurons had expressed tyrosine hydroxylase. By 15th postnatal day tyrosine hydroxylase neurons appeared mature and were scattered, suggesting a well-formed neuropil. NCAM and L1 reaction was seen as a peripheral rim in most of the cells on the fifth postnatal day. The reaction was mainly in relation to the large cells and more extensive on the 15th day. Thereafter on the 25th day, activity was negligible. Large neurons demonstrated strong reactivity for NCAM and L1 during early post-transplantation days. After 30 days only smaller cells were reactive, many of which could be identified as neurons. Strong reaction for these molecules was present only until 60 days, though faint reaction could be detected even on the 90th day. These observations indicate that the growth promoting molecules, the type seen in the neonatal period, can be detected normally only until the neurons mature. Prolonged expression of these molecules by the grafted neurons indicate delay in the maturation of these cells due to absence of adequate target sites for synaptic connections. Some of the smaller cells expressing these molecules after 30 days of transplantation could be astroglia, either proliferating or reactive.

Item Type:Article
Source:Copyright of this article belongs to International Brain Research Organization.
Keywords:Substantia Nigra; Intrastriatal Graft; Growth-promoting Molecules; Tyrosine Hydroxylase
ID Code:13626
Deposited On:12 Nov 2010 15:14
Last Modified:02 Jun 2011 04:52

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