Verma, Shashikala ; Bhatnagar, Rakesh (2014) MoxT toxin of Bacillus anthracis exhibits sequence specific ribonuclease activity Biochemical and Biophysical Research Communications, 450 (2). pp. 998-1004. ISSN 0006-291X
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Official URL: http://doi.org/10.1016/j.bbrc.2014.06.092
Related URL: http://dx.doi.org/10.1016/j.bbrc.2014.06.092
Abstract
MoxXT module of Bacillus anthracis encodes MoxX, a labile protein and MoxT, a ribonuclease. However, mechanism of cleavage of RNA by MoxT has not been explored till date. In the present study, we have demonstrated that MoxT is a sequence specific ribonuclease which recognizes UACAU sequence in ss RNA and cleaves between U and A. Moreover, cleavage of RNA requires 2' OH group of first residue, i.e. U of UACAU RNA sequence. An interesting finding which makes it distinct from the other MazF family toxins was also observed, i.e. its ability to cleave RNA in DNA-RNA hybrid.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 133771 |
| Deposited On: | 30 Dec 2022 06:58 |
| Last Modified: | 30 Dec 2022 06:58 |
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