Generation of active immunotoxins containing recombinant restrictocin

Rathore, Dharmendar ; Batra, Janendra K. (1996) Generation of active immunotoxins containing recombinant restrictocin Biochemical and Biophysical Research Communications, 222 (1). pp. 58-63. ISSN 0006-291X

Full text not available from this repository.

Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1006/bbrc.1996.0697

Abstract

Restrictocin, a toxin produced by the fungusAspergillus restrictus,is a potent inhibitor of eukaryotic protein synthesis. Recombinant restrictocin was made inEscherichia coliand purified to homogeneity in large amounts. The recombinant protein was found to be poorly immunogenic in mice with low toxicity, when injected intraperitoneally. Two immunotoxins were constructed by coupling the recombinant restrictocin to an antibody to the human transferrin receptor, using a cleavable and a stable linkage. The immunotoxins so generated showed specific cytotoxic activity toward receptor bearing cells in tissue culture. Immunotoxin with a cleavable linkage, however, was more active than that containing a stable linkage. Restrictocin appears to be a promising candidate to be developed as a chimeric toxin for targeted therapy.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:13348
Deposited On:11 Nov 2010 08:17
Last Modified:17 May 2011 09:16

Repository Staff Only: item control page