Pahari, Susanta ; Pari, Amal ; Chattopadhyay, D.J. (1996) Periplasmic Expression of Biologically Active Vesicular Stomatitis Virus Phosphoprotein P inEscherichia coli Protein Expression and Purification, 7 (4). pp. 384-388. ISSN 10465928
Full text not available from this repository.
Official URL: http://doi.org/10.1006/prep.1996.0057
Related URL: http://dx.doi.org/10.1006/prep.1996.0057
Abstract
Overexpression of a clone of vesicular stomatitis virus phosphoprotein P (New Jersey serotype) using T7 promoter withphoAleader sequence and a simpler two-step purification procedure of the expressed protein has been developed. The purified protein retains its ability to activate the transcription reaction. Comparative transcriptional assay using the protein P purified from periplasmic space and from cytosol (in the form of inclusion body) ofEscherichia coliestablishes the fact that the former is 10 times more efficient than the latter in activating the transcription reactionin vitro.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Academic Press |
| ID Code: | 132509 |
| Deposited On: | 19 Dec 2022 06:07 |
| Last Modified: | 19 Dec 2022 06:07 |
Repository Staff Only: item control page
