Downregulation of CCR5 on activated CD4 T cells in HIV-infected Indians

Dubey, Shweta ; Khalid, Mohammad ; Wesley, Clement ; Khan, Samir Ali ; Wanchu, Ajay ; Jameel, Shahid (2008) Downregulation of CCR5 on activated CD4 T cells in HIV-infected Indians Journal of Clinical Virology, 43 (1). pp. 25-31. ISSN 1386-6532

Full text not available from this repository.

Official URL:

Related URL:


Background: HIV infection in India is unique as it occurs predominantly by CCR5-utilizing isolates that exhibit no co-receptor switch. Objectives: To study HIV-1 co-receptor dynamics on T cells and monocytes following viral infection. Study design: HIV co-receptor expression was evaluated by flow cytometry on various cell subsets in HIV-infected Indians and in vitro in human peripheral blood mononuclear cells infected with CCR5- or CXCR4-utilizing HIV-1. Transfection of the T cell line CEM-CCR5 (which expresses CD4, CCR5 and CXCR4) with HIV-1 Nef or Vpu expression vectors, or treatment with recombinant soluble gp120 from CCR5- and CXCR4-tropic HIV-1, was carried out to determine their effects on co-receptor expression. Results: Indian HIV patients had fewer CD4+CCR5+ T cells and CCR5-expressing activated CD4+ T cells, but higher CXCR4-expressing activated CD4+ T cells compared with controls. Expression of CCR5 was not different on monocytes in HIV patients as compared to controls. The CCR5 downregulation on T cells was HIV infection specific and was governed by the co-receptor-utilization phenotype of the virus. The Nef and soluble gp120 proteins induced CCR5 downregulation, the latter in a co-receptor-utilization phenotype specific manner. Conclusions: The HIV-1 co-receptor dynamics in Indian patients is distinct from western patients and depends upon the virus surface protein. We propose this to be a viral survival strategy.

Item Type:Article
Source:Copyright of this article belongs to Pan American Society for Clinical Virology.
Keywords:HIV; CCR5; CXCR4; Co-receptor downregulation; India
ID Code:13135
Deposited On:11 Nov 2010 06:47
Last Modified:17 Feb 2011 06:15

Repository Staff Only: item control page