Hepatitis B virus surface (S) transactivator with DNA-binding properties

Saxena, Alka ; Durgapal, Hemlata ; Chaudhuri, Vaishali ; Jameel, Shahid ; Panda, Subrat Kumar (2000) Hepatitis B virus surface (S) transactivator with DNA-binding properties Journal of Medical Virology, 61 (1). pp. 1-10. ISSN 0146-6615

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Official URL: http://www3.interscience.wiley.com/journal/7100568...

Related URL: http://dx.doi.org/10.1002/(SICI)1096-9071(200005)


Chronic infection with hepatitis B virus (HBV) in humans is strongly linked to the development of hepatocellular carcinoma (HCC). Activation of growth-regulatory genes may play a crucial role in carcinogenesis. Proto-oncogene expression has been shown to be higher in HCC tissue with integrated HBV DNA than in the normal liver. Earlier, we showed that the 3' end of the HBV major surface gene (S) (426-855 nucleotides of the S region) is a transactivator of the X promoter-enhancer regulatory element in co-transfection experiments. This region expresses a truncated carboxy terminal S protein extending from amino acid residues 102 to 226. In this study, the truncated S protein (trc-S) was examined for its enhancing activity on several viral and cellular regulatory elements. The results indicate that trc-S activates rous sarcoma virus long terminal repeat (LTR), human T-lymphotropic virus 2 LTR, human immunodeficiency virus 1 LTR, and the c-jun and c-fos promoters. Electrophoretic mobility shift assays carried out to investigate its DNA-binding properties established that trc-S binds to HBV X promoter and oligonucleotides representing binding sites for the AP1 and TFIID transcription factors. The specificity of this interaction was confirmed by using competition experiments and supershift assays. These experiments suggest that trc-S is a transactivator of several cellular and viral promoters and that this activity is mediated by direct interaction with DNA.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Hepatitis B Virus; Transactivator; Hepatocellular Carcinoma; Cellular and Viral Promoters; Electrophoretic Mobility Shift Assay
ID Code:13088
Deposited On:11 Nov 2010 04:56
Last Modified:23 Feb 2011 05:33

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