Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii

Gayathri, P. ; Banerjee, M. ; Vijayalakshmi, A. ; Azeez, S. ; Balaram, H. ; Balaram, P. ; Murthy, M. R. N. (2007) Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii Acta Crystallographica Section D: Biological Crystallography, 63 (2). pp. 206-220. ISSN 0907-4449

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The crystal structure of a recombinant triosephosphate isomerase (TIM) from the archaeabacterium Methanocaldococcus jannaschii has been determined at a resolution of 2.3 Å using X-ray diffraction data from a tetartohedrally twinned crystal. M. jannaschii TIM (MjTIM) is tetrameric, as suggested by solution studies and from the crystal structure, as is the case for two other structurally characterized archaeal TIMs. The archaeabacterial TIMs are shorter compared with the dimeric TIMs; the insertions in the dimeric TIMs occur in the vicinity of the tetramer interface, resulting in a hindrance to tetramerization in the dimeric TIMs. The charge distribution on the surface of the archaeal TIMs also facilitates tetramerization. Analysis of the barrel interactions in TIMs suggests that these interactions are unlikely to account for the thermal stability of the archaeal TIMs. A novelty of the unliganded structure of MjTIM is the complete absence of electron density for the loop 6 residues. The disorder of this loop could be ascribed to a missing salt bridge between residues at the N- and C-terminal ends of the loop in MjTIM.

Item Type:Article
Source:Copyright of this article belongs to International Union of Crystallography.
Keywords:Triosephosphate Isomerase (TIM); Methanocaldococcus jannaschii; Tetrameric TIM; Tetartohedral Twinning; Disordered Loop 6
ID Code:1272
Deposited On:04 Oct 2010 07:57
Last Modified:16 May 2016 12:25

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