G-Quadruplex-Specific Cell-Permeable Guanosine–Anthracene Conjugate Inhibits Telomere Elongation and Induces Apoptosis by Repressing the c-MYC Gene

Abstract

We herein report a cell-membrane permeable molecular probe ADG, prepared by conjugating guanosine with anthracene selectively interacts with c-MYC quadruplex over other promoter and telomeric quadruplexes as well as duplex DNA. NMR spectroscopy suggests that ADG interacts with terminal G-quartets as well as with the nearby G-rich tract (G13-G14-G15 and G8-G9-G10) of c-MYC quadruplex. In vitro cellular studies indicate that ADG represses c-MYC expression by stabilizing its promoter G-quadruplex and alters c-MYC related cellular events. ADG suppresses hTERT and BCL2 gene expressions, inhibits elongation of telomere length and activates apoptotic cascades in cancer cells.