Madhav, Maganti S. ; Plaha, Parikshit ; Singh, Nagendra K. ; Sharma, Tilak R. (2009) Molecular Characterization of a Genomic Fragment Containing Pi-kh Gene from the Genomic Library of Indica Rice Line Tetep Journal of Phytopathology, 157 (5). pp. 322-324. ISSN 0931-1785
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Official URL: http://doi.org/10.1111/j.1439-0434.2008.01487.x
Related URL: http://dx.doi.org/10.1111/j.1439-0434.2008.01487.x
Abstract
Identification of full length genes along with upstream regulatory elements is important to understand its expression. Here, we report preparation of high titre genomic library and identification of a genomic clone containing Pi-kh gene with its complete upstream and downstream sequences from the rice blast resistant line Tetep. Structural analysis of protein revealed that Pi-kh has a central nucleotide binding site domain, leucine-rich repeats domain and a unique zinc-finger domain. Comparative analysis of Pi-kh protein sequence showed 64% and 45% similarity with the protein sequences of rice blast resistance genes Pi-b and Pi-ta, respectively.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley & Sons, Inc. |
| ID Code: | 124597 |
| Deposited On: | 26 Nov 2021 10:19 |
| Last Modified: | 26 Nov 2021 10:20 |
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