Oxygen-dependent and -independent mechanisms of renal injury in experimental ascending pyelonephritis

Gupta, Ashish ; Sharma, Nidhi ; Sharma, Bal K. ; Sharma, Saroj ; Ganguly, Nirmal K. (1996) Oxygen-dependent and -independent mechanisms of renal injury in experimental ascending pyelonephritis FEMS Immunology & Medical Microbiology, 13 (1). pp. 35-42. ISSN 0928-8244

Full text not available from this repository.

Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...

Related URL: http://dx.doi.org/10.1111/j.1574-695X.1996.tb00213.x


Pyelonephritis is the most common urinary tract infection affecting females of all age groups. Despite concerted efforts the mechanism of renal injury in pyelonephritis is not clearly understood. In the present study we have made an attempt to characterise the mediators of inflammatory insult in an experimental model of ascending pyelonephritis. Mice infected with Escherichia coli O6:K13:H1 were sacrificed at 2, 7 and 14 days post-infection. Luminol-dependent chemiluminescence response, NADPH oxidase, acid phosphatase, β:-glucuronidase and N-acetyl-β:-d-glucosaminidase activities were monitored in circulating as well as renal phagocytic cells in order to determine the role of reactive oxygen species and lysosomal enzymes in genesis of renal injury. We have demonstrated that reactive oxygen species are generated at the initiation of infection and the levels increase progressively during the course of infection. While intracellular release of lysosomal enzymes was seen in all groups, extracellular release was primarily observed at 7 and 14 days post-infection only. The results indicate that while reactive oxygen species play a significant role in tissue injury during all stages of infection, lysosomal enzyme release in extracellular milieu augments tissue destruction at later stages only.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Microbiological Societies.
Keywords:Reactive Oxygen Species; Acid Phosphatase; β-Glucuronidase; N-acetyl-β-d-glucosaminidase; Pyelonephritis
ID Code:12202
Deposited On:10 Nov 2010 04:39
Last Modified:31 May 2011 10:28

Repository Staff Only: item control page