Kaul, Pankaj ; Malla, N. ; Kaur, Sukhbir ; Mahajan, R. C. ; Ganguly, N. K. (2000) Evaluation of a 200-kDa amastigote-specific antigen of L. donovani by enzyme-linked immunosorbent assay (ELISA) for the diagnosis of visceral leishmaniasis Transactions of the Royal Society of Tropical Medicine and Hygiene, 94 (2). pp. 173-175. ISSN 0035-9203
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00359...
Abstract
A purified 200-kDa antigenic fraction from Leishmania donovani axenic amastigotes was evaluated by ELISA for the detection of antibody response in visceral leishmaniasis (VL) patients, post kala-azar dermal leishmaniasis (PKDL) patients and controls, for the diagnosis of visceral leishmaniasis. A positive antibody response to the 200-kDa amastigote fraction and to Leishmania amastigote soluble antigen (LASA) was found in 29 (96.6%) and 30 (100%) confirmed VL patients, respectively, by the use of ELISA. However, only 1 (10%) out of 10 PKDL patients had detectable antibody response to 200-kDa fraction while all the 10 (100%) PKDL patients exhibited an immune response to LASA. Therefore, use of the 200-kDa antigenic fraction for the detection of antibody response in an ELISA follow-up (post treatment) of VL patients may have prognostic significance, and it may also be useful for differentiating active VL and PKDL.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Royal Society of Tropical Medicine and Hygiene. |
| Keywords: | Visceral Leishmaniasis; Post Kala-azar Dermal Leishmaniasis; Serodiagnosis; Prognosis; ELISA; Antigens; India |
| ID Code: | 12140 |
| Deposited On: | 10 Nov 2010 04:17 |
| Last Modified: | 31 May 2011 10:21 |
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