Construction of a restriction map of bacteriophage T3 DNA

Sarkar, Probir ; Adhya, Samit ; Musich, Phillip ; Maitra, Umadas (1980) Construction of a restriction map of bacteriophage T3 DNA Gene, 12 (1-2). pp. 161-163. ISSN 0378-1119

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/037811...

Related URL: http://dx.doi.org/10.1016/0378-1119(80)90027-X

Abstract

A restriction endonuclease cleavage map of bacteriophage T3 DNA has been constructed. The enzymes used and, within parentheses, the number of their cleavage sites on T3 DNA are: HindIII (1), XbaI (1), BglII (1), KpnI (2), MboI (9), and HpaI (17). The size and the relative location of each fragment have been established, defining an accurate physical map of T3 DNA.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:HpaI, MboI, BglII, KpnI, HindIII, and XbaI Restriction Endonucleases; T4 Polynucleotide Kinase; Exonuclease III; Gel Electrophoresis; E. coli
ID Code:12111
Deposited On:10 Nov 2010 04:29
Last Modified:31 Dec 2010 04:35

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