Chromosome profile of Leishmania donovani: interstrain and interspecific variations

Ghosh, Siddhartha Sankar ; Mukerjee, Sandeep ; Adhya, Samit (1998) Chromosome profile of Leishmania donovani: interstrain and interspecific variations Journal of Biosciences, 23 (3). pp. 247-254. ISSN 0250-5991

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The genome of Leishmania donovani AG83, a virulent strain causing kala-azar, was resolved into 29 chromosomal bands by pulsed field gel electrophoresis (pFGE) under standardized conditions. Comparison of the karyotype with those of other strains and species revealed variations. By Southern hybridization, specific genes were localized to individual chromosomes. Twenty-two copies of β -tubulin genes are located on band 27 (1.63 Mb); minor copies are present in band 16 (850 kb) and band 9 (650 kb). A β-tubulin related nontranscribed locus was isolated from a genomic library and shown to contain repetitive sequences hybridizing throughout the genome. Single chromosomes contain multicopy clusters of gp63 and rnini-exon-derived RNA genes, but interspecific variations were observed in each case. The results emphasize the importance of using a standard reference strain of Leishmania donovani for coordinated genome mapping of this clinically important organism.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Leishmania donovani; Karyotype; Pulse Field Gel Electrophoresis; Gene Localization
ID Code:12080
Deposited On:10 Nov 2010 04:43
Last Modified:16 May 2016 21:29

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