Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction

Adhya, Samit ; Chatterjee, Mitali ; Hassan, Md. Quamarul ; Mukherjee, Sandeep ; Sen, Sandeep (1995) Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction Transactions of the Royal Society of Tropical Medicine and Hygiene, 89 (6). pp. 622-624. ISSN 0035-9203

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Abstract

Samples from 39 patients with symptoms suggestive of early visceral leishmaniasis were independently assayed by microscopy of tissue smears, enzyme-linked immunosorbent assay (elisa) and polymerase chain reaction (PCR) of blood deoxyribonucleic acid. Of these patients, 19 were confirmed as positive or negative by all 3 tests; 11 patients (28%) negative by smear were positive by elisa and PCR; and 7 (18%) were positive by PCR alone. These results demonstrate the high sensitivity of the non-invasive PCR and, to a smaller extent, elisa, in the early diagnosis of visceral leishmaniasis.

Item Type:Article
Source:Copyright of this article belongs to Royal Society of Tropical Medicine and Hygiene.
Keywords:Visceral Leishmaniasis; Leishmania Donovani; Diagnosis; Elisa; Pcr; Microscopy; India
ID Code:12041
Deposited On:10 Nov 2010 04:49
Last Modified:26 May 2012 14:15

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