B cell responses to a peptide epitope. II: Multiple levels of selection during maturation of primary responses

Tuteja, Renu ; Agarwal, Anshu ; Vijayakrishnan, Lalitha ; Nayak, Bishnu P. ; Gupta, Satish K. ; Kumar, Vijay ; Rao, Kanury V. S. (1997) B cell responses to a peptide epitope. II: Multiple levels of selection during maturation of primary responses Immunology and Cell Biology, 75 (3). pp. 245-252. ISSN 0818-9641

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Official URL: http://www.nature.com/icb/journal/v75/n3/abs/icb19...

Related URL: http://dx.doi.org/10.1038/icb.1997.38

Abstract

This report analyses murine primary humoral recognition of a linear domain (MEP 17-31) within a 100 amino acid polypeptide. MEP-I. An analysis of the early primary IgM response revealed that MEP 17-31 presented at least two distinct domains for pre-immune B cell recognition represented by MEP-1 residues 19-23 and 26-28. However, subsequent maturation into an IgG response saw an exclusive selection for the anti-MEP 19-23 component with loss of all alternate specificities. The IgM response to MEP 19-23 was oligoclonal and composed of diverse paratope phenotypes as evidenced by varied heavy chains of immunoglobulin V-D-J combinations and CDR3 sequences. In contrast to the oligoclonality of IgM niAb. the mature IgG response to MEP 19-23 appeared to derive predominantly from a single progenitor. It therefore appears that maturation of primary humoral responses to polypeptide antigens involves two distinct levels of selection. While there is selection for a restricted subset of the initially induced antibody fine-specificities, progression of the response also entails a reduction in clonal heterogeneity of B cells responding to the dominant epitope.

Item Type:Article
Source:Copyright of this article belongs to Nature Publishing Group.
Keywords:Clonal Selection; Peptide Epitope; Primary Response
ID Code:12031
Deposited On:10 Nov 2010 05:01
Last Modified:16 May 2016 21:26

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