Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood

Dada, Rima ; Kumar, Rakesh ; Shamsi, M. B. ; Kumar, Rajeev ; Kucheria, Kiran ; Sharma, Raj K. ; Gupta, Satish K. ; Gupta, Narmada P. (2007) Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood Asian Journal of Andrology, 9 (5). pp. 720-722. ISSN 1008-682X

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1745-...

Related URL: http://dx.doi.org/10.1111/j.1745-7262.2007.00274.x


Aim: To determine if Yq microdeletion frequency and loci of deletion are similar in two tissues (blood and sperm) of different embryological origin. Methods: The present study included 52 infertile oligozoospermic cases. In each case, DNA was isolated from blood and sperms and polymerase chain reaction (PCR) microdeletion analysis was done from genomic DNA isolated from both the tissues. The PCR products were analyzed on a 1.8% agarose gel. PCR amplifications found to be negative were repeated at least three times to confirm the deletion of a given marker. Results: Only 1 case harbored microdeletion in blood DNA, whereas 4 cases harbored microdeletion in sperm DNA. Conclusion: The frequency of Yq microdeletions is higher in germ cells as compared to blood. As the majority of infertile couples opt for assisted reproduction procreation techniques (ART), Yq microdeletion screening from germ cells is important to understand the genetic basis of infertility, to provide comprehensive counseling and most adapted therapeutics to the infertile couple.

Item Type:Article
Source:Copyright of this article belongs to Asian Society of Andrology.
Keywords:Assisted Reproductive Techniques; Infertility; Semen; Genomic DNA; Yq Screening
ID Code:12024
Deposited On:10 Nov 2010 05:04
Last Modified:02 Jun 2011 11:10

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