Novel method for screening pigeonpea varieties for globulin content by radioimmunoassay

Mawal, Madhumalti R. ; Mawal, Yogesh R. ; Guru Row, T. N. ; Ranjekar, P. K. (1988) Novel method for screening pigeonpea varieties for globulin content by radioimmunoassay Biotechnology Techniques, 2 (1). pp. 53-56. ISSN 0951-208X

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Official URL: http://www.springerlink.com/index/l65h701778278wj2...

Related URL: http://dx.doi.org/10.1007/BF01874208

Abstract

A rapid and sensitive radioimmunoassay (RIA) is described for screening a large number of pigeonpea varieties for quantitation of globulin content. This method employs precipitation of iodine-labelled pigeonpea globulin by polyethylene glycol and measuring the labelled antigen-antibody complex. Among the 56 varieties screened, Gwalior-3 was found to contain the highest globulin content. RIA when used to quantitate the pigeonpea globulin levels during post anthesis revealed that maximum globulin is present between 24 to 28 days after flowering.

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ID Code:11933
Deposited On:13 Nov 2010 13:32
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