Tripathi, A. K. ; Mishra, B. M. (1996) Isolation and characterization of Azospirillum lipoferum locus that complements Rhizobium meliloti dctAanddctBmutations Canadian Journal of Microbiology, 42 (5). pp. 503-506. ISSN 0008-4166
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Official URL: http://doi.org/10.1139/m96-067
Related URL: http://dx.doi.org/10.1139/m96-067
Abstract
A DNA probe containing the structural gene for dicarboxylate transport (dct A) of Rhizobium meliloti hybridized strongly with the fragments of Azospirillum lipoferum genomic DNA. A genomic library of A. lipoferum was screened for the dct A gene by complementation of a dct A mutant of Rhizobium meliloti. A recombinant cosmid, p37D, capable of restoring growth of the dct A mutant on dicarboxylates was isolated and found to hybridize to the dctA probe. The ability of p37D to complement the dct B mutant of R. meloliti indicated that dct A and dct B genes in A. lipoferum may be organized adjacent to each other
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Canada Institute for Scientific and Technical Information. |
| ID Code: | 119211 |
| Deposited On: | 09 Jun 2021 05:27 |
| Last Modified: | 09 Jun 2021 05:27 |
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