Chakravortty, D ; Nanda Kumar, K S (1997) Microplate Assay For Detection Of Bacterial Contamination In Tissue Culture Media Indian Journal of Experimental Biology, 35 (1). pp. 86-88. ISSN 0019-5189
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Official URL: http://nopr.niscair.res.in/handle/123456789/39
Related URL: http://dx.doi.org/PMID: 18345591
Abstract
In the present study, a rapid and simple colorimetric technique has been described to determine the presence of bacteria in tissue culture medium used in animal cell culture. The microplate assay is based on utilization of MTT [3(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] by bacteria resulting in formation of formazan crystals which can be measured colorimetrically. Contaminated medium, a standard gram-negative and gram-positive bacteria produce formazan from MTT which is related to the bacterial load. The assay has utility in screening tissue culture reagents to detect the presence of bacteria.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| ID Code: | 118473 |
| Deposited On: | 21 May 2021 09:32 |
| Last Modified: | 21 May 2021 09:32 |
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