Bacterial transformation using micro-shock waves

Divya Prakash, G. ; Anish, R.V. ; Jagadeesh, G. ; Chakravortty, Dipshikha (2011) Bacterial transformation using micro-shock waves Analytical Biochemistry, 419 (2). pp. 292-301. ISSN 0003-2697

Full text not available from this repository.

Official URL: http://doi.org/10.1016/j.ab.2011.08.038

Related URL: http://dx.doi.org/10.1016/j.ab.2011.08.038

Abstract

Shock waves are one of the most competent mechanisms of energy dissipation observed in nature. We have developed a novel device to generate controlled micro-shock waves using an explosive-coated polymer tube. In this study, we harnessed these controlled micro-shock waves to develop a unique bacterial transformation method. The conditions were optimized for the maximum transformation efficiency in Escherichia coli. The maximum transformation efficiency was obtained when we used a 30 cm length polymer tube, 100 μm thick metal foil, 200 mM CaCl2, 1 ng/μl plasmid DNA concentration, and 1 × 109 cell density. The highest transformation efficiency achieved (1 × 10−5 transformants/cell) was at least 10 times greater than the previously reported ultrasound-mediated transformation (1 × 10−6 transformants/cell). This method was also successfully employed for the efficient and reproducible transformation of Pseudomonas aeruginosa and Salmonella typhimurium. This novel method of transformation was shown to be as efficient as electroporation with the added advantage of better recovery of cells, reduced cost (40 times cheaper than a commercial electroporator), and growth phase independent transformation.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Novel Transformation Method; Shock Wave; Micro-Shock Wave; Bacterial Transformation.
ID Code:118411
Deposited On:21 May 2021 06:00
Last Modified:21 May 2021 06:00

Repository Staff Only: item control page