Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella

Allam, Uday Sankar ; Krishna, M. Gopala ; Sen, Minakshi ; Thomas, Rony ; Lahiri, Amit ; Gnanadhas, Divya Prakash ; Chakravortty, Dipshikha (2012) Acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella Virulence, 3 (2). pp. 122-135. ISSN 2150-5594

Full text not available from this repository.

Official URL: http://doi.org/10.4161/viru.19029

Related URL: http://dx.doi.org/10.4161/viru.19029

Abstract

During the course of infection, Salmonella has to face several potentially lethal environmental conditions, one such being acidic pH. The ability to sense and respond to the acidic pH is crucial for the survival and replication of Salmonella. The physiological role of one gene (STM1485) involved in this response, which is upregulated inside the host cells (by 90- to 113-fold) is functionally characterized in Salmonella pathogenesis. In vitro, the ΔSTM1485 neither exhibited any growth defect at pH 4.5 nor any difference in the acid tolerance response. The ΔSTM1485 was compromised in its capacity to proliferate inside the host cells and complementation with STM1485 gene restored its virulence. We further demonstrate that the surface translocation of Salmonella pathogenicity island-2 (SPI-2) encoded translocon proteins, SseB and SseD were reduced in the ΔSTM1485. The increase in co-localization of this mutant with lysosomes was also observed. In addition, the ΔSTM1485 displayed significantly reduced competitive indices (CI) in spleen, liver and mesenteric lymph nodes in murine typhoid model when infected by intra-gastric route. Based on these results, we conclude that the acidic pH induced STM1485 gene is essential for intracellular replication of Salmonella.

Item Type:Article
Source:Copyright of this article belongs to Informa UK Limited.
Keywords:Salmonella Containing Vacuole; Acid Shock Proteins; Acid Tolerance Response; Acidification; Competitive Indices; Epithelial Cells.
ID Code:118358
Deposited On:20 May 2021 12:37
Last Modified:20 May 2021 12:37

Repository Staff Only: item control page