New visible light excitable donor–acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani

Raghuvanshi, Ashutosh ; Kumar Jha, Ajay ; Kathuria, Manoj ; Priya Awasthi, Bhanu ; Purohit, Deepak ; Mitra, Kalyan ; Goel, Atul (2017) New visible light excitable donor–acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani Journal of Materials Chemistry B, 5 (14). pp. 2580-2587. ISSN 2050-750X

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Official URL: http://doi.org/10.1039/C6TB03257E

Related URL: http://dx.doi.org/10.1039/C6TB03257E

Abstract

In order to address the existing limitations of the commercially available fluorescent probe CMAC (7-amino-4-chloromethylcoumarin), a new series of highly fluorescent donor–acceptor 7-hydroxy-coumarin derivatives was prepared and these derivatives were used as vacuole specific fluorescent probes for live cell imaging of unicellular parasitic protozoa L. donovani promastigotes and yeast cells S. pombe and S. cerevisiae. The synthesized 7-hydroxy-coumarins exhibited interesting photophysical properties and have advantages such as excitation in the visible region, good water solubility, photo-stability, good quantum yield and low cytotoxicity.

Item Type:Article
Source:Copyright of this article belongs to The Royal Society of Chemistry.
ID Code:117972
Deposited On:07 May 2021 09:46
Last Modified:07 May 2021 09:46

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